- Title
- Reproductive failure in mice expressing transgenic follicle-stimulating hormone is not caused by loss of oocyte quality
- Creator
- Upton, Dannielle H.; Walters, Kirsty A.; McTavish, Kirsten J.; Holt, Janet; Handelsman, David J.; Allan, Charles M.
- Relation
- NHMRC.1008160 http://purl.org/au-research/grants/nhmrc/1008160
- Relation
- Biology of Reproduction Vol. 98, Issue 4, p. 491-500
- Publisher Link
- http://dx.doi.org/10.1093/biolre/ioy015
- Publisher
- Oxford University Press
- Resource Type
- journal article
- Date
- 2018
- Description
- Human female reproductive aging features declining ovarian follicle reserve and oocyte quality, and rising levels of circulating follicle-stimulating hormone (FSH). We determined the effects of elevated FSH on oocyte-embryo development in mature mice exhibiting premature infertility caused by progressively rising transgenic human FSH (TgFSH) levels. Oocyte-embryo developmental competence and quality were examined using oocyte maturation and aneuploidy rates, biomarkers of oocyte quality, and reciprocal embryo transfers assessed for implantation and pregnancy. In vitro maturation suggested that TgFSH exposure only hindered oocyte developmental competence in old females, as significantly more oocytes from ≥12-month-old TgFSH females remained at germinal vesicle stage compared with age-matched control oocytes. Aneuploidy rates were equivalent in oocytes from aging TgFSH compared with wildtype females. Cumulus cell expression levels of candidate biomarker Inhba, Egfr, and Rgs2 transcripts were elevated in associated aneuploid vs euploid oocytes from both TgFSH and wildtype females. In vivo, embryos transferred from subfertile 6-month-old TgFSH females to wildtype recipients yielded normal implantation rates and more pups born compared with controls. Transfer of wildtype embryos rescued the fertility of 6-month-old TgFSH-recipient females, although pup birth weight was reduced in TgFSH vs wildtype recipients. Our current findings show that elevated FSH had minimal disruption of either embryo developmental capacity or uterine function when examined in isolation, and the subfertility of TgFSH female mice was not caused by altered oocyte aneuploidy or quality.
- Subject
- transgenic mice; follicle-stimulating hormone; oocyte quality; embryonic development; aging; aneuploidy; developmental competence
- Identifier
- http://hdl.handle.net/1959.13/1405779
- Identifier
- uon:35549
- Identifier
- ISSN:0006-3363
- Language
- eng
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